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文献和实验Immunocytology using the HA epitope
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein
Immunofluorescence Analysis Using Epitope-Tagged Proteins: In Vitro System
in crossreactivity with several proteins because of similar amino acid sequences. The problem of low titer antibody and poor antigenicity can be overcome by marking the proteins with an epitope “tag.” The most widely used tags in prokaryotic and eukaryotic cells
Multiple tandem epitope tagging for enhanced detection of protein expressed in mammalian cells
Epitope tagging is a valuable tool for quick detection, isolation, and analysis of protein-protein interaction, without prior knowledge of the target protein. The FLAG epitope tag, one of the most widely used tags, is an eight amino acid peptide
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