thermo特约代理商|26161 Pierce™ ChIP

Chromatin Immunoprecipitation (ChIP)

for ChIP PCR or real-time PCR primers Phenol:chloroform (pH 8.0) Phosphate-buffered saline (PBS) (1X) Protease inhibitors (Roche) Protein A-agarose/salmon sperm DNA beads (Millipore) or Protein G-agarose/salmon sperm DNA beads

新版ChIP分析试剂盒：EZ ChIP™

过程和troubleshoot 试剂盒规格: 22 ChIP操作 试剂盒组成 * DNA Purification Spin Columns and Collection Tubes * Protein G Agarose / Salmon Sperm DNA * Anti-RNA Polymerase II,clone CTD448 * Control PCR Primers * Normal Mouse IgG * Bind,Wash

Chromatin IP (CHIP assay)

and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time:      2 x 1 ml CHIP lysis buffer      2 x 1 ml CHIP