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文献和实验Isolation of micro-RNA (miRNA)
Adapted from the Instruction manual of Stratagene''s Micro RNA Isolation Kit (Catalog #200344) Overview There are two main methods for isolating RNA: phenol-based extraction and silica matrix or glass fiber filter (GFF
Beta-gal staining of eukaryotic cells in vitro
for 35 mm dish; I use room temp. tissue culture PBS without Ca++ or Mg++) - beware that some cell types, eg. NIH-3T3, BALB/C-3T3 or morphologically transformed cells are easily sloughed off the surface after 2nd wash. 2. Aspirate. 3. Fix cells
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
) TrypLE Express (Gibco) Equipment Aspirator tube (Sigma-Aldrich) Bunsen burner Capillary tubes (glass; Fisher Scientific) Centrifuge Dish (glass-bottom, 50-mm diameter [40-mm diameter glass]) (Intracel)
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