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Two dimensional peptide mapping

  This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol

Peptide Inhibition ELISA Protocol

Dilute specific and non-specific peptides to 200 mg/ml in PBT. In a microtiter plate, mix equal volumes of the peptide dilutions and the MAb supe.  Add an equal volume of PBT and MAb as the control.  Incubate 1 hour at 37

peptide fingerprint mapping

,with the development of ESI-MS and MALDI-MS.Peptide mass fingerprinting uses the peptide masses obtained by digestion to search the protein and DNA databases,to find proteins that show a similar theoretical digest pattern (see eg .,Shevchenko et al.1996a and b