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文献和实验Array-Based Spectral SPR Biosensor: Analysis of Mumps Virus Infection
Spectral SPR biosensor is a useful system for a rapid analysis of protein arrays, as the biosensor with a fiber optic spectrometer can be easily aligned with the reflected light from protein arrays. The spectral SPR biosensor was constructed
Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
The immobilization procedure to a biosensor surface has a major influence on the measurement results. To characterize the immobilization onto various biolayers, the interaction of DNA anti-thrombin aptamer with the protein thrombin was used
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