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文献和实验Chromosomal double strand breaks (DSBs) can be repaired by a number of mechanisms that result in diverse genetic outcomes. To examine distinct outcomes of chromosomal DSB repair, a panel of human cell lines has been developed that contain GFP
Two-hybrid analysis of genetic
protein sequences suggested residues that may be important for specific interactions with certain Cdis.Second,Cdi3,Drosophila Cyclin D,interacted much more strongly with human Cdk4 than with any of the other Cdks in the panel including the Drosophila Cdks
Two-hybrid analysis of genetic regulatory networks
, and results in fusion of the two haploids to form a diploid yeast strain. Thus, an interaction can be determined by measuring activation of a two-hybrid reporter gene in the diploid strain. As described below, interaction mating has been used
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