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文献和实验Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
Bromodeoxyuridine Staining of Mammary Tissue
The following is a procedure we have used and modified for detecting DNA synthesis in cells of mouse mammary glands I. BrdU Treatment Mice were injected i.p. with 0.01 mL/g bromodeoxyuridine
Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
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