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DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

and oxidation reactions require transfer of an electron , which is facilitated by electron acceptors of the proper redox potential (Cotson and Holt, 1958). The ferric and ferrous ions included in most X-gal reaction buffers provide this function (Lojda, 1970

Oligodeoxynucleotide Containing S‐Functionalized 2′‐Deoxy‐6‐Thioguanosine: Facile Tools for Base‐Selective and Site‐Specific Internal Modification of

oligonucleotides play a significant role for genomic research. Modified nucleosides, such as with a fluorescent dye, can be obtained by chemical synthesis. Site?specifically modified long nucleic acids are obtained by ligation of chemically modified short

常用试剂配制－3

. It is commercially available from Pierce Chemical Company, Rockford, Illinois as Protein Assay Reagent, Cat. # 23200. It contains methanol and solubilizing agents and is very rel iable. If you wish to make your own, dissolve 100 mg of Coomasie