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上海玉博生物科技有限公司
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文献和实验using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
15. DPBS Without Calcium or Magnesium 16. TRIzol® LS Reagent 17. SuperScript® VILO™ cDNA Synthesis Kit 18. AccuPrime™ SuperMix I 19. Mouse Anti-Tra1-60 Antibody 20. Mouse Anti-Tra1-81 Antibody 21. Mouse Anti-SSEA
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