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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
during the extended incubation period. Crosses are made on petri dishes for specific purposes, as when shot asci are to be collected. But plates are more prone to contamination and desiccation than tubes, and less compact for incubation and storage. 2. Media
Live imaging with Drosophila tissue culture cells
) (see note 3): Culture dishes or slides with coverslip bottom are used. Some providers produce round culture dishes (e.g. Willco Wells B.V.®) or 8-well or single well culture slides (e.g. Nunc™ or ibidi®, see figure 2); To follow
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
V 4. ASAP using a pasteur pipette transfer cells from curvette A to a 10 ml tube containing 4 mls medium, then plate 5 x 1 ml cells onto five 10cm dia petri dishes containing NeoPMEF's and 10 ml medium. Label plates 4-8. B to a 10 ml tube containing 1ml
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