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Isolation of RNA from Difficult Tissues

    Isolation of RNA from Difficult Tissues The often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and tissues rich in protein, DNA

Early development of primary motor neurons and somites in Zebrafish Embryos

  Background: Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is a popular species for studying vertebrate development. Cleavage in the Zebrafish only occurs in the blastodisc,a region

RNA-isolation (TRIZOL method)

  RNA-isolation (TRIZOL method)     Isolation of RNA from whole worms using TRIZOL reagent (Gibco-BRL). 1) Wash worms from a 60 mm plate with 1 ml DEPC-treated water. 2) Spin down worms at 4000xg in microfuge