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文献和实验and then store at 4°C until use (if less than two weeks). For the vinyl plates, wash three times with washing solution, dispel liquid by slapping on paper towels and then cover and store at -70°C. These plates are good for at least 6 months. 2. Wash tubes
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
solution ABA 5mg/1ml EtOH, ditto cycloheximide, make 5mg/ml stock in EtOH, use 40ul/20ml aleurone solution chloramphenicol, make 50mg/ml stock in EtOH, use 40ul/20ml aleurone solution HORMONES & INHIBITORS For protoplasts, dilute
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