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文献和实验Disulfide Conjugation of Peptides to Oligonucleotides and Their Analogs
of oligonucleotides and their analogs is being studied widely towards improving the delivery of oligonucleotides into cells. Amongst the many possible routes of conjugation, the disulfide linkage has proved to be the most popular. This reversible linkage
-translational modifications like disulfide bonds. • ALiCE® Lysate • Tubes 特点和优势: • 高产量：前所未有的 3 mg/mL • 简易操作：一步法 • 快速: 48小时收获超高产量 • 高灵活性:表达困难蛋白, 抗体、抗原、有毒的蛋白，等. • 验证:已在超过500种蛋白验证 • 翻译后修饰(e.g. 折叠、二硫键、糖基化 等) ALiCE® Kit无细胞蛋白质表达试剂盒: SKU-Pack
Co-Expression of human DNA primase p49-p58 subunits
) •3mM BME (to prevent disulfide formation,Ni beads are compatible w/low concs.of BME,but NOT DTT.) Wash buffer: •1% Triton X-100 •50mM Tris,pH 8.0 •300mM NaCl (Qiagen recommends at least 300mM NaCl to prevent ionic interactions w/Ni-NTA beads.NaCl
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