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上海玉博生物科技有限公司
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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
II. REFERENCES •Transfectol-LS Mammalian Transient transfection Kit. GeneChoice; TR10v2 KT-011106 III. EQUIPMENT, MATERIALS, AND REAGENTS • CO2 incubator, Forma Scientific incubator • BioSafety Cabinet, • Orbital Shaker, Forma
Large-scale immunofluorescence
overnight7 at 4o C with very gentle shaking. Wash cells once with PBS, once with PBS plus 0.1% NP40, and then again with PBS. Resuspend cells in 30 ul of PBS plus 0.1% BSA containing the secondary antibody8 , and incubate for 4 hours at room
atio n o f rib o so m es. Mol. Cell Biol. 1 0 , 6 2 57-6263. 30. K im , Y. , M ls a , D. , M o n zin g o , A. F . , R e a d y , M . P . , F r a n k e l, A. , a n d R o b e r tu s , J. D. ( 1 9 9 2 ) S tr u c tu r e o
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