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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
II. REFERENCES •Transfectol-LS Mammalian Transient transfection Kit. GeneChoice; TR10v2 KT-011106 III. EQUIPMENT, MATERIALS, AND REAGENTS • CO2 incubator, Forma Scientific incubator • BioSafety Cabinet, • Orbital Shaker, Forma
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
II. REFERENCES •Transfectol-LS Mammalian Transient transfection Kit. GeneChoice; TR10v2 KT-011106 III. EQUIPMENT, MATERIALS, AND REAGENTS • CO2 incubator, Forma Scientific incubator • BioSafety Cabinet, • Orbital Shaker, Forma
Large-scale immunofluorescence
overnight7 at 4o C with very gentle shaking. Wash cells once with PBS, once with PBS plus 0.1% NP40, and then again with PBS. Resuspend cells in 30 ul of PBS plus 0.1% BSA containing the secondary antibody8 , and incubate for 4 hours at room
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