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文献和实验Methods for Creating Mutations in C. elegans That Extend Lifespan
The principle of commonly used methods to create mutations in the nematode Caenorhabditis elegans (C. elegans ) is straightforward. In general, worms are exposed to a dose of mutagen resulting in DNA damages and mutations. Screening
Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice
Calorie restriction is the most powerful method currently known to delay aging-associated disease and extend lifespan. Use of this technique in combination with genetic models has led to identification of key metabolic regulators of lifespan
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
II. REFERENCES •Transfectol-LS Mammalian Transient transfection Kit. GeneChoice; TR10v2 KT-011106 III. EQUIPMENT, MATERIALS, AND REAGENTS • CO2 incubator, Forma Scientific incubator • BioSafety Cabinet, • Orbital Shaker, Forma
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