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文献和实验must be maintained in such a way that they persist in their differentiated state. While many hepatocyte culture methods exist, the two-dimensional collagen “sandwich” system combined with a serum-free medium, supplemented with physiological glucocorticoid
Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing
, and more specifically hepatocyte-based in vitro models, are currently being applied. A major problem, however, related to the use of hepatocytes and their cultures is their limited viability, which is associated with the loss of phase I and phase II biotransformation
Isolation and Long-Term Maintenance of Adult Rat Hepatocytes in Culture
Long-term and stable hepatocyte culture systems have a wide variety of uses, both in basic science and in the development of hepatocyte-based applications. In most cases, long-term cultures of hepatocytes are superior to traditional cultures
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