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上海玉博生物科技有限公司
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文献和实验Agrobacterium growth and transformation
-DNA transfer, but has no functional T-DNA region of its own. Grow at 28-30℃. Store as glycerol stock (800 ml of fresh overnight culture + 200 μl sterile 80% glycerol) at -80℃.Transformation of A. tumefaciens with plasmid DNA (binary vector system)Two methods
Agrobacterium growth and transformation
Growth and storage of Agrobacterium tumefaciens Strain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampicin on plates or in liquid media for selection. GV3101 is sensitive to kanamycin
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
Orbital Shaker • Water Bath, VWR Scientific • 500 ml Vented Cap Erlenmymer Flask, • 125 ml Vented Cap Erlenmymer Flask, Corning product • Pro293S-CDM, 1 L (Cambrex Biosciences, Cat 12-765Q) • Penicillin-Streptomycin, Invitrogen • Transfection
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