Seracare特约代理商|5270-0023 Strept

Dual- and Triple-Color Fluorospot

labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml

蛋白芯片制作与应用－操作流程

) and GST-FRB and (His)6-FKBP12 were produced recombinantly in Escherichia coli. IKBalpha and (His)6-FKBP12 were labeled with Cy3 and Cy5, respectively, using monofunctional reactive dye from Amersham Pharmacia Biotech (Newark, NJ) and following

二 抗

花青染料（Cy2,Cy3,Cy5）：Cy2比FITC更稳定，且在表面荧光显微镜下，Cy2的荧光FITC更强。Cy3和Cy5比大多数荧光基团更亮，更稳定，背景更弱。二者常在共聚焦显微实验中作多标记。但Cy5的缺点是不能用表面荧光显微镜观察，因此通常用具有远红外检测器的共聚焦显微镜观察。 TRITC,RRX,TR:TRITC经常和FITC一起用于双标记实验中，也可以使用RRX或TR，但TR会产生较高的背景。在使用装有氪氢灯的激光共聚焦扫描显微镜作三标记实验时，RRX尤其有用