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上海玉博生物科技有限公司
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文献和实验at step 12. Dehydrate quickly RESULTS : --Collagen - Red --Nuclei - Blue-Black --Background - Yellow --Elastin Black SOLUTIONS: Picro-Sirius red 0.1 Sirius red F3B in saturated Aqueous Picric Acid Weigert
Elastic Picro Sirius Red 1. Bring sections to water 2. Acidified potassium permanganate 2 minutes 3. Wash in water 4. 1% Oxalic acid 1 minute 5. Wash in water 6. Wash in 70% alcohol (optional) 7. Stain in Weigert's
Dissolve Proteinase K (Sigma Catalog No. P-4914) in dH2O for a concentration of 20mg/ml. 1% BSA/PBS 1g Fraction V BSA in 100ml 1x PBS. Mix in a beaker with low heat. Aliquot in 15ml tubes and store at -20°C. Hematoxylin 75ml Gill's
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