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上海玉博生物科技有限公司
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文献和实验2-D Polyacrylamide Gel Electrophoresis
: Use electrophoresis grade reagents to prepare the following solutions: A: 50 ml IEF Lysis Buffer 1.Add 21 g urea to 35 ml HPLC-grade H2O to a 50 ml Falcon tube (final concentration 7 M). 2.Vortex vigorously for several minutes. 3.Add: 7.6 g
they are well-mixed up. Place 11.6 ul of master mix into each tube. Make sure you pipette the mix to the bottom of the tube as I don't have any way of spinning these strips. Keep strips on ice as much as possible. 6. Place the appropriate amount of DNA and water
Here we describe a LightCycler-based real-time PCR for quantitative detection of EBV DNA in clinical samples such as unfractionated whole blood, serum, or plasma. This assay is based on amplification of a highly conserved 213-bp region
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