RPI特约代理商|145530A Micro-Centrif
uge Tubes, 1.5ml Capacity, Graduated 100µl, Non-Stick, Assorted, 500 per Case 一包500个- ￥3000
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- 145530A
- 2025年07月12日
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文献和实验. 2. Reaction: a. Heat at 100 deg C for 10" to deNature : 15 µl (50-250ng) DNA (in TE or ddH2O) b. Cool quickly on ice (1-2") c. Add: 2 µl 10X hexa nt mix 2 µl 10X dig/dNTP mix 1 µl Klenow* (5u/µl) d. Incubate at 37 deg C
Preparation of total RNA from whole blood collected in PAXgene tubes
yields of total RNA.use 95% ethanol; 100% ethanol often contains benzene (bad for arrays).8.Apply 700 µl sample to the PAXgene column sitting in a 2 ml processing tube, and centrifuge for 1 min at ³ 8000 x g ( ³ 10,000 rpm). Place the PAXgene column
at 100 deg C for 10' to denature: 15 µl (50-250ng) DNA (in TE or ddH2 O) b. Cool quickly on ice (1-2') c. Add: 2 µl 10X hexa nt mix 2 µl 10X dig/dNTP mix 1 µl Klenow* (5u/µl) d. Incubate at 37 deg C >1 hr
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