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Ethidium Bromide Decontamination

N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide.   Decontamination of solutions > 0.5mg/ml This method reduces the mutagenic activity

Clean-up for Ethidium Bromide

Caution: EtBr is a powerful mutagen and is moderately toxic. Wear gloves when working. When EtBr conc. is >0.5 mg/ml: 1.Add sufficient water to reduce the concentration of EtBr to 2.Add 1 volume of 0.5 M KMnO4. Mix, add 1 volume of 2.5 N HCl

Decontamination of Ethidium Bromide Solutions and Surfaces

has been validated for EtBr solutions of less than 0.5mg/ml in water, TBE buffer, MOPS buffer and CsCl gradients. For solutions greater than 0.5 mg/ml, dilute to 0.5 mg/ml with water. - Liquid waste: Add 1 part decontamination solution