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上海玉博生物科技有限公司
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
mRNA In Situ Hybridization with Nick-Translated Probes
cells are incubated in 4X SSC/0.25% BSA/2ug/ml anti-digoxigenin antibody for 60 min. in a humid chamber at room temperature in the dark. Coverslips are then rinsed in 4X SSC (1 X 15 min.) at room temperature, 4X SSC/0.1% Triton X-100 (1 X 15 min
MINICHROMOSOME MICROTUBULE BINDING ASSAY
(containing 0.1mM DTT, 100mM NaCl, 5% glycerol and 1mg/ml BSA, but NO PMSF!). These are the PELLET FRACTIONS. NOTE: Ultimately, PELLETs will be 2x concentrated relative to SUPs. Add 250ul of 2x ASSAULT buffer (containing tRNA and øX174) to all samples
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