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文献和实验Estimation of Fat in Milk Samples
Introduction Milk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is necessary to know for casein/fat ratio normalization. It is also important
Blocking of unwanted non-specific staining
Blocking endogenous biotin Can be done with commercially available kits or by buying the isolated components of the kits, free biotin and free avidin. Can also be done with simpler reagents as below. 1- Wash twice in TBS 0.05M pH
be available))) for 5 minutes 5% non fat milk in BN or BI buffer for 10 minutes (c) detection: 50µl of 5% non fat milk in BN or BI buffer containing 4µg FITC-Avidin (Vector Labs, DCS grade) for 60 minutes (d) wash in BN or BI buffer, 2 x
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