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文献和实验In vitro culture of embryonic lungs
). Isolated endoderm will not survive without growth factor added to the culture medium. Solutions Tyrode-Ringer's Solution, pH 7.6-7.7 component g/liter NaCl 8.0 KCl 0.3 NaH2PO4.5H2O 0.093 KH2PO4 0.025 NaHCO
In vitro culture of embryonic lungs
for three to five days (see accompanying protocol). Isolated endoderm will not survive without growth factor added to the culture medium. Solutions Tyrode-Ringer's Solution, pH 7.6-7.7 component g/liter NaCl 8.0 KCl 0.3 NaH2
Whole genome amplification protocol for ChIP-chip
, vortex or mix by pipetting and quick spin if necessary; Incubate in thermal cycler as follows: 16°C for 20' (cycler should be precooled to this temperature) 24°C for 20' 37°C for 20' 75°C for 5' 4°C hold; Quick spin
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