vector laboratories 代理商|H-1000

用DAPI进行细胞染色

:• Wash the cells twice with PBS• Incubate with 1: 750 of stock DAPI for 5 min in dark• Wash 2 x 5 min with PBS in dark• Rinse with water for a few seconds• Air dry at RT in dark• Mount with antifade solution (Vectashield, Co#: H-1000, Vector Lab)5 µl

免疫荧光操作（共聚焦显微镜）-Immunofluorescence Protocol for Confocal Microscopy

¨ Vectashield (Vector Laboratories H-1000 and H-1200 with DAPI) antifade medium gives better antifade protection than SlowFade Light but is hard on the cells; I see lots of membrane blebbing on both fixed B cells in suspension and on fixed adherent

Immunofluorescence / Confocal Microscopy Protocol

Vectashield (Vector Laboratories H-1000 and H-1200 with DAPI) antifade medium gives better antifade protection than SlowFade Light but is hard on the cells; I see lots of membrane blebbing on both fixed B cells in suspension and on fixed adherent melanoma cell