vector laboratories特约代理商|H-150
0-10 VECTASHIELD HardSet Antifade Mounting Medium with DAPI 10ML- ￥3000
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- TRIM33-110H
- 2025年07月16日
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文献和实验免疫荧光操作（共聚焦显微镜）-Immunofluorescence Protocol for Confocal Microscopy
¨ Vectashield (Vector Laboratories H-1000 and H-1200 with DAPI) antifade medium gives better antifade protection than SlowFade Light but is hard on the cells; I see lots of membrane blebbing on both fixed B cells in suspension and on fixed adherent
/0.5 ml in H2O and stocked at �20 in the darkStaining with antifade mounting solution:• Wash the cells twice with PBS• Mount with antifade solution with DAPI (Vectashield, Co#: H-1200，Vector Lab, 1.5 µg/ml) at 1:3 dilution with Vectashield H-1000
Immunofluorescence / Confocal Microscopy Protocol
Vectashield (Vector Laboratories H-1000 and H-1200 with DAPI) antifade medium gives better antifade protection than SlowFade Light but is hard on the cells; I see lots of membrane blebbing on both fixed B cells in suspension and on fixed adherent melanoma cell
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