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文献和实验Western Blotting with Monoclonal Ant
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. Polyacrylamide Gel
Western Blotting with Monoclonal Antibodies
for 30 minutes with agitation, changing the wash buffer every 3? minutes. Dilute the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies).
【翻译】Development trends for monoclonal antibody cancer therapeutics
7 and patients frequently developed antibodies to the mouse-derived proteins, which limited their utility8, 9. In addition, only certain murine mAb isotypes have been shown to effectively bind to and activate elements of the human immune
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