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文献和实验Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
/negative genes can be introduced to study the effect of signal interference on cells. This basic protocol is concerned with the application of GFP as an indicator of transfection in chicken embryos. Lipofectin® Reagent (Life Technologies, catalog
BLOCK-iT Fluorescent Oligo as RNAi Transfection Control
-iT™ Fluorescent Oligo for Cationic Lipid-Mediated Transfection You may use the BLOCK-iT™ Fluorescent Oligo (20 µM or 1 mM stock) with any cationic lipid-based transfection reagent suitable for delivery of Stealth™ RNAi, siRNA, or Dicer-generated siRNA
E-Z 96® Viral RNA Protocol with Centrifugation
from step 6 (including any precipitate) to each well of the HiBind® RNA plate. 8. Seal the HiBind® RNA plate with new sealing film. Load the HiBind® RNA plate with 2ml 96-well plate into the plate holder, and place the whole assembly into the rotor
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