Logos Biosystems特约代理商|L12001 L

Counting ES cell Chromosomes

for four hours in the presence of colcemid (3.125 µl of 10 µg/ml colcemid (Gibco # 15212-012) per ml of medium). 2ml per 35 mm and 4ml per 60 mm. 3) Remove media and place it in a 15 ml Falcon tube. Wash with trypsin, and trypsinize with 1.5 ml trypsin.

Cell Cultures

transfer vessels. Each day students will observe these transfer cultures with an inverted phase contrast microscope, and remove aliquots for cell counting with a hemacytometer. Simultaneously, they will check on the viability of the cells through a dye

Cell Sorting By FACS

-cell suspension. If you removed adherent cells by trypsinization, etc., resuspend them well (observe under microscope). Cell clumps should also be removed by passing cells through a cell-strainer. Note that there are reagents such as Accutase™