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文献和实验Fluorometric Quantitation of RNA or DNA
up enough reagent for the samples plus a blank and at least one control. Large cuvettes require 1.5 mL/sample Mini-cuvettes need 100 µL/sample. Aliquot 1.5 mL (large cuvette) or 0.1 mL (microcuvette) of either Höchst
Preparation of cytospin from single cell suspension.
the slide, paper and cuvette without disarranging. Carefully detach the cuvette and the paper without damaging the fresh cytospin. Is very important to hold firmly together glass slide and cuvette when extracting from metal holder. Mark the area
Turn on machine and wait for it to warm up Wash glass cuvettes thoroughly before use and if 2 are used then make sure they are a ‘matching’ pair. For Spec. in Dave Andersons bay then press ‘goto ?’ type in ‘260’ press ‘enter
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