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上海玉博生物科技有限公司
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文献和实验) which is also very sensitive; ALL solutions must be ICE COLD so place on ice before harvesting cells. All solutions containing sucrose must be MADE UP FRESH on the day; Remember to add protease inhibitors (whichever your lab uses) to all solutions
GFP gene encoding Green Fluorescent Protein mutant p11 URA3 URA3 gene from S. cerevisiae tet Tetracycline resistance gene res Tn3 site for resolution
HIGH RESOLUTION GENETIC FOOTPRINTING
flasks 50 mg/ml carbenicillin (10.5 ml) IPTG (595 mg), Sigma Various buffer components (make sure there is DTT, reduced glutathione, autoclaved 80% glycerol, protease inhibitors, etc.) lysozyme protease inhibitors: PMSF, pepstatin A, leupeptin
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