sigma特约代理商|11982 Tenax® 多孔聚合物吸

Screening Colonies by Hybridization with Radiolabeled Probe

, nitrocellulose filter size) nitrocellulose filters (petri dish size, usually Ø82mm) vacuum oven (80°C) Eppendorf tube with black ink, needle stuck through lid Reagents: 10% SDS (optional) (dissolve 50 g SDS in 500 ml

Analys of Genomic DNA by Southe

hybridization bottles hybridization oven at 42, 37, 68℃ Pyrex glass trays Salmon Testes DNA (Sigma D-9156), 10.4 mg/ml 1 % Ethidium bromide stock solution (10 µg/µl) Agarose

Electrophoresis of PCR products with Sunrise gel apparatus

: 3 g H2 O 270 mls 10X TA 30 mls Screw cap partially on.