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文献和实验Preparing Colloidal Gold for Electron Microscopy
after they have been made. To make 100 ml of gold sol, two stock solutions have to be prepared. Solution A 80 ml distilled water and 1 ml 1% aqueous gold chloride. Solution B 4 ml 1% tri
Southern Blotting: DNA Transfer
: a. Large sheet of plastic wrap b. Two pieces blot paper (precut to same size as gel) soaked in 20x SSC c. Gel (wells-side down) d. Presoaked nylon e. One piece blot paper soaked in 5x SSC f. 10-15 pieces of dry blot paper h. Wrap whole sandwich
Southern Blotting:DNA Transfer
7.0 Hybridization Sol'n: 5x SSC 0.5% (w/v) Blocking Reagent 0.1% (w/v) N-lauroylsarcosine, Na-salt 0.02% (w/v) SDS 50% (w/v) formamide *Blocking reagent does not dissolve rapidly. Heat to ~50-70℃
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