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文献和实验MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol
from DiOC1(5). The example below is a protocol for labeling with an annexin V–Alexa Fluor® 488 conjugate and propidium iodide. 1) After step 3 (above), wash cells once by adding 2 mL of warm phosphate-buffered saline or other buffer to each tube
-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. 主要试剂 Alexa Fluor® 488 annexin V Propidium iodide PBS [Details
cells treated with 1 µM camptothecin. The externalized phosphatidylserine, a characteristic of early-stage apoptotic cells, was detected with Alexa Fluor 488 annexin V (A13201). The late-stage apoptotic and necrotic cells were stained with propidium
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