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文献和实验Introduction to the EMSA (Gel Shift) Technique
Chemiluminescent EMSA Kit, Product # 20148 ) and a kit to facilitate labeling DNA with biotin (Biotin 3´ End DNA Labeling Kit, Product # 89818 ). The LightShift EMSA Kit utilizes Pierce’s patented SuperSignal Chemiluminescent Detection Technology to offer detection
EMSA using ds Oligonucleotides
EMSA using ds Oligonucleotides Solutions 10X Annealing Buffer 200 mM Tris 8.0 200 m l 1M Tris pH 8.0 10 mM EDTA 8.0 20 m l .5M EDTA pH 8.0 500 mM NaCl 100 m l 5M NaCl 280 m l Q store at room
Procedure for GenEluteTM Plasmid Miniprep Kit(质粒小量
to each miniprep column and centrifuge at 12000 × g for 30 seconds to 1 minute. Discard the flow-through liquid. Note : The Column Preparation Solution maximizes binding of DNA to the membrane resulting in more consistent yields. 5. Load cleared
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