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- 详细信息
- 文献和实验
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- 供应商:
上海玉博生物科技有限公司
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文献和实验I) or fluorography (3H, 14C, and 35S). Method II A. Reagents IMP Buffer 50 mM Tris-acetate, pH 7.5 150 mM NaCl 1% (v/v) Triton X-100 1% (w/v) deoxycholate 0.1% (w/v) SDS 4 mM AEBSF 1 µg/ml aprotinin1 10 µg/ml leupeptin1 0.02% (w/v) NaN3 Notes: 1) Prepare
Measurement of NO and NO Synthase
tubing 10, 25, 50, and 100 µl gas‐tight Hamilton syringes Support Protocol 1: Preparation
) = 0.37g, H2 O = 1.0ml NaOH (1N) = 14µl; 2. solubilize in the boiling water bath (to heat ~1-3'; until pH will drop to ~7.0). PEG H(OCH2 CH2 )n OH; (store at 4o C). Conc. %(w/w) 10ml 50ml 100ml 150ml
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