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上海玉博生物科技有限公司
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文献和实验SECTIONS 4µm paraffin wax sections SOLUTIONS 1. STOCK ALCOHOLIC SALT SOLUTION --Saturated sodium chloride in 80% alcohol 2. WORKING SOLUTION --Stock solution (1) 50cm3 --1% Sodium hydroxide 0.5cm3 --Mix, filter
Recombineering/Lambda red-mediated gene replacement
Overview Single-gene knockouts using λ red system, adapted from Datsenko and Wanner paper. The goal of this protocol was to create an endA (endonuclease I) knockout, but obviously it can be adapted to any gene. The knocked-out gene
Sample Protopcol for Two-Step Carbodiimide Coupling of Protein to MagPlex™ Magnetic Carboxylated Microspheres Microspheres should be protected from prolonged exposure to light throughout this procedure. 1.Resuspend
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