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武汉科昊佳生物科技有限公司
ABI荧光定量PCR A25742武汉科昊佳生物科技有限公司现货特价促销!
| 品 名 | 品牌 | 货号 | 规格 | 促销价 | ||
| PowerUp™ SYBR™ Green Master Mix | ABI | A25742 |
|
900 | ||
| PowerUp™ SYBR™ Green Master Mix | ABI | A25777 | 5 x 5 mL | 询价 | ||
| PowerUp™ SYBR™ Green Master Mix | ABI | A257778 |
|
询价 |
规格
| Detection Method: | SYBR |
| For Use With (Equipment): | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Reaction Speed: | Fast or Standard |
| PCR Method: | qPCR |
| Sample Type (General): | DNA |
| Number of Reactions: | 500 reactions |
| Polymerase: | Dual-Lock™ Taq DNA Polymerase |
| Product Line: | PowerUP™, SYBR® |
| Product Size: | 5 mL |
| Shipping Condition: | Wet Ice |
内容及储存
The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 1 X 5 mL tube, sufficient for 500 20-µL reactions (10 µL Master Mix per reaction).Store at 2–8°C.
描述
PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments
Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.
Tight reproducibility in CTs over a wide dynamic range
PowerUp™ SYBR® Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR® Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).
UDG included for carryover contamination control
The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR® Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.
Stability of pre-assembled reactions
The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR® Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR® Green Master Mix across numerous workflow scenarios1.
Broad instrument compatibility
PowerUp™ SYBR® Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments2. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. For optimal results, recommended primer concentrations are 300–800 nM.
Learn more about PowerUp™ SYBR® Green Master Mix >
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文献和实验相关实验
SYBR Green Quantitative PCR Protocol
12μl of master mix for each well, plus some excess1 Rxn: 10μl SYBR Green Mix 52 Rxn: 520μl SYBR 0.4μl Forward Primer (10μM stock) 20.8μl F
REAL TIME PCR WITH SYBR GREEN I 建议PROTOCOL
1、反应体系 25ulCocktail 20 ul: Primer FP 1 ul + Primer RP 1u + 2 * SYBR GREEN I MIX 12.5 + H2O 5.5 ul cDNA 5 ul2、Primer 浓度，需要摸索，从200nM到700nm等，设置不同浓度。3、每个引物浓度，从well 1到12设置4个样本，阳性cDNA 1和2，NTC以及H2O，做triplicate。4、若用ABI7000或7700，所有的PCR条件可设置成一致的，减少麻烦，当然，引物
ABI7000 use notes ，131k，word文档 http://www.wi.mit.edu/CMT/protocols/ABI7000RT_vCMT1.doc Primer Express ,96页，PDF格式 http://www.wi.mit.edu/CMT/protocols/ABI7000RT_vCMT1.doc SYBR® Green PCR
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ABI荧光定量PCR A25742 PowerUp™ SYBR™ Green Master Mix
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