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T-RFLP的优缺点

in order to facilitate the loading of the sample. Gel purification is then performed by separating the PCR products by electrophoresis on agarose gel, excising the band of proper size and recovering the PCR product with a gel purification kit (Qiagen, MOBIO

T-RFLP技术的优缺点

by separating the PCR products by electrophoresis on agarose gel, excising the band of proper size and recovering the PCR product with a gel purification kit (Qiagen, MOBIO, Promega) following manufacturer's recommendations. If nonspecific PCR products