QIAGEN凯杰特约代理商|55114 QIAamp Cir

文献速递：全面液体分析循环肿瘤DNA和蛋白质生物标志物为评估预后带来新的可能

，并且可以与其他策略相结合，提供更好的评估。 其中，为了验证ctDNA动态与HCC进展中的肿瘤负荷相关性，该研究团队提取并分析了患者在临床过程中收集的连续血浆样本中的ctDNA。采用了QIAamp Circulating Nucleic Acid Kit+ QIAseq cfDNA All-in-One Kit进行血浆游离DNA提取与文库构建，并结合全基因组测序与靶向测序技术来获得突变等位基因频率的相关性（图A）。 图A. HCC进展过程中血浆样本中体细胞SNVs和CNVs的动态变化。在患者H1607的临床

E.Z.N.A. Cycle-Pure Kit Spin Protocol

from 100bp to 500bp gives yields of 60%- 90%. The ratio of (A )/280 (A ) is an indication of nucleic acid purity. Alternatively, yield (as well as quality) can sometimes be best determined by agarose gel/ethidium bromide electrophoresis.

Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)

I digestion mix as following table. Note: If total nucleic acid (DNA and RNA) is desired, skip this DNase I digestion steps (step 14-21) and proceed step 17 for isolating both DNA and RNA. Note: If total nucleic acid (DNA and RNA) is desired