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文献和实验Isolation of RNA from Difficult Tissues
, and offer troubleshooting techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion's
Isolation of Cell-Free DNA from Maternal Plasma Using Manual and Automated Systems
system using the MagNA Pure LC instrument (Roche Diagnostics). Use of the methods described here will help to ensure maximum yield and purity of cell-free fetal DNA extracted from maternal plasma samples for downstream analyses.
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
. For Total Nucleic acid isolation, proceed step 8-12. For RNA and DNA isolation, proceed step 13-25. Total Nucleic acid Isolation: 8. Add equal volume (400 μL) of isopropanol. Mix throughly by vortexing for 30 seconds. Incubate the tube at room
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