abcam特约代理商|9050 Anti-Histone H

ChIP 实验面面观，博士师兄吐血总结推荐

、TRANSFAC、ECR browser 等） 进行预测，然后确保设计的引物的扩增产物能够横跨这个预测的潜在结合位点。而对于组蛋白修饰（如 Tri-Methyl-H3-K9） 的 ChIP 实验，对引物的扩增范围要求则相对宽松，只要扩增产物能够位于启动子区域或基因转录区域即可。为了方便大家使用，CST 提供很多已经经过验证的检测多个基因的 ChIP primer，请前往 CST 官方网站直接输入 ChIP primer 进行搜索或联系 CST 技术支持（4006-473-287） 获取信息。对于 ChIP

Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization

. When this experiment is performed with an antibody recognizing an invariant domain of H3 (H3core; Abcam #AB1791), dilution of the input chromatin improves the ChIP efficiency. These data show that the relation between ChIP signal and chromatin input depends

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey