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文献和实验ml D. 20×Leu 25.0ml E. Agar 10.00g F. ddH2O → 500.0ml 90-100mm)f铺20-25块平板( 11. 挑取上述固体培养基上生长的含有目的DNA(X)的pLexA-X(+)或空载pLexA(-)单菌落，分别接种SD/Gal/Raf/-Ura，-His，-Trp，-Leu固体诱导培养板，30℃倒置培养3-5天。 SD/Gal/Raf/-Ura，-His，-Trp，-Leu固体诱导
Materials (Solutions are all available from the media room) 200ml bottle of 2x SD 200ml bottle of 4% agar -- make sure to sign it out 40% glucose CSM minus the relevant marker(s), as powder Stack
EXAMINING THE FUNCTION OF PROTEINS AND PROTEIN NETWORKS WITH THE YEAST TWO-HYBRID SYSTEM
2 at a sufficiently low level to be used for an interactor hunt. However, if one were to then attempt to thoroughly screen a library of 3 x 106 individual cDNAs by plating over 3 x 106 CFU onto the leu- selection plates, at least 3000 colonies would form
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