biorad特约代理商|1610737 2x Laemmli

Preparation of protein extracts for western blot

with 2 ml 50 mM Tris,pH 7.5.,blot away excess liquid and freeze pellet on dry ice. 5.To frozen pellet add 0.2g 0.5 mm glass beads and 50 µl 2% SDS. 6.Vortex 90-120 sec full speed. 7.Place in boiling water bath for 3 min. 8.Add 100 µl 2x Laemmli sample

BOILING PROTEIN PREP

bath for 3 min. 8.Add 100 µl 2x Laemmli sample buffer(or use 75μl of 3X Laemmli sample buffer). 9.Place in boiling water bath for 1 min. 10.Spin briefly in Tomy (at room temp--15 sec,3K),remove liquid with a ml pippetteman tip,and transfer to 1.5 ml

Nucleolar Isolation Protocol

on to a polylysine-coated slide (BDH Cat no: 406/0178/00), and air dry the spot. Rehydrate the slide in PBS for 5 min before carrying out a standard immunostaining procedure. - To separate nucleolar proteins on a gel, either resuspend directly in Laemmli SDS sample