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文献和实验Silencing Gene Expression with Dicer-Generated siRNA Pools
it has been difficult and time-consuming to develop a cellular model deficient in one or more proteins. The discovery of RNA interference (RNAi) has made loss-of-function studies relatively quick and easy and amenable to high-throughput formats (1 –3 ). Double
Discovery of Posttranscriptional Regulatory RNAs Using Next Generation Sequencing Technologies
Next generation sequencing (NGS) has revolutionized the way by which we engineer metabolism by radically altering the path to genome-wide inquiries. This is due to the fact that NGS approaches offer several powerful advantages
The characterization of molecular alterations specific to cancer facilitates the discovery of predictive and prognostic biomarkers important to targeted therapeutics. Alterations critical to cancer therapeutics include copy number alterations
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