- 询价
- ES-006-B
- 2026年05月29日
- Stem Cell Culture
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Stem Cell Culture
- 宿主：
0
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
0
- 规格：
500 mL
1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:
| Wells | _ | Amount/Well |
|---|---|---|
| 96 | 100 μL | |
| 48 | 300 μL | |
| 24 | 0.5 mL | |
| 12 | 1.0 mL | |
| 6 | 1.5-2.0 mL |
2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.
3. Remove the gelatin solution and discard.
4. Immediately add cells to the dish.
Note: Do not allow the dishes to dry before adding cells.
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
- Cell Culture Supplements
- Attachment Factors
- Stem Cell Reagents
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详细描述见链接：http://www.millipore.com/catalogue/item/ES-006-B
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
The technique described here is a slight modification (March 1997) of methods presented in: Nagy A., J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practical Approach. (ed. A. Joyner). IRL Press at Oxford
ES Cell Culture and Manipulation
ribonucleosides 1/100 volume ß-ME stock (stock is 7µl ß-ME in 10ml DMEM) 1:1000 dilution of LIF (add 500 µl 720LIFD cell conditioned media to 500ml media) Thawing frozen vials of ES cells Thaw vial rapidly at 37°C, rinse outside of vial
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE
glutamine with 4.5 g/L Glucose. Store at 4� Cytosystems 500 ml Cat. No. 11.016.0500V Non essential amino acids 100x Cytosystems 100 ml Cat No. 21-145-0100V. Store at 4� Penicillin - streptomycin (5,000 1U/ml, 5,000 ug/ml) Cytosystems 100
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