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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human coronavirus(HCoV-NL63)Spike/S1 Protein(S1 Subunit,His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg
蛋白名称:Coronavirus spike蛋白, Coronavirus spike protein
蛋白构建:A DNA sequence encoding the human coronavirus (HCoV-NL63) spike protein (S1 Subunit) (APF29071.1) (Cys19-Val717) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Cys 19
蛋白分子量:The recombinant human coronavirus (HCoV-NL63) spike protein (S1 Subunit, His Tag) consists of 710 amino acids and predicts a molecular mass of 78.8 kDa.
蛋白NP号:APF29071.1
蛋白氨基酸序列:Cys19-Val717
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Bertoglio F, et al. SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface.Nature communications, PubMed ID: 33707427
3, Ravindran R, et al. Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses.PloS one, PubMed ID: 34242356
4, Mazhari R, et al. SARS-CoV-2 Multi-Antigen Serology Assay.Methods and protocols, PubMed ID: 34698238
5, Geanes ES, et al. Cross-reactive antibodies elicited to conserved epitopes on SARS-CoV-2 spike protein after infection and vaccination.Scientific reports, PubMed ID: 35444221
6, Denninger V, et al. Microfluidic Antibody Affinity Profiling Reveals the Role of Memory Reactivation and Cross-Reactivity in the Defense Against SARS-CoV-2.ACS infectious diseases, PubMed ID: 35352558
7, Tasić N, et al. Study of V2CTx-MXene Based Immunosensor for Sensitive Label-Free Impedimetric Detection of SARS-CoV-2 Spike Protein.ACS applied materials & interfaces, PubMed ID: 38814245
8, Brustoloni CJ, et al. Systematic Study of Various Functionalization Steps for Ultrasensitive Detection of SARS-CoV-2 with Direct Laser-Functionalized Au-LIG Electrochemical Sensors.ACS applied materials & interfaces, PubMed ID: 39231012
Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay
229E and OC43 of human coronavirus (HCoV). An alternative indirect immunoperoxidase assay (IPA) is herein described for the detection and titration of these viruses. Susceptible cells are inoculated with serial logarithmic dilutions of samples in a 96
A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
The recent discoveries of novel human coronaviruses, including the coronavirus causing SARS, and the previously unrecognized human coronaviruses HCoV-NL63 and HCoV-HKU1, indicate that the family Coronaviridae harbors more members
, we described the identification of a novel human coronavirus, HCoV-NL63, with the use of the VIDISCA method.
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