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- Sino Biological
- 北京
- 40600-V08H
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human coronavirus(HCoV-NL63)Spike/S1 Protein(S1 Subunit,His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg
蛋白名称：Coronavirus spike蛋白, Coronavirus spike protein
蛋白构建：A DNA sequence encoding the human coronavirus (HCoV-NL63) spike protein (S1 Subunit) (APF29071.1) (Cys19-Val717) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Cys 19
蛋白分子量：The recombinant human coronavirus (HCoV-NL63) spike protein (S1 Subunit, His Tag) consists of 710 amino acids and predicts a molecular mass of 78.8 kDa.
蛋白NP号：APF29071.1
蛋白氨基酸序列：Cys19-Val717
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Bertoglio, F; et al. SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface. Nature communications, PMID: 33707427
3, Ravindran, R; et al. Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses. PloS one, PMID: 34242356
4, Mazhari, R; et al. SARS-CoV-2 Multi-Antigen Serology Assay. Methods and protocols, PMID: 34698238
5, Geanes, ES; et al. Cross-reactive antibodies elicited to conserved epitopes on SARS-CoV-2 spike protein after infection and vaccination. Scientific reports, PMID: 35444221
6, Denninger, V; et al. Microfluidic Antibody Affinity Profiling Reveals the Role of Memory Reactivation and Cross-Reactivity in the Defense Against SARS-CoV-2. ACS infectious diseases, PMID: 35352558
7, Tasić, N; et al. Study of V2CTx-MXene Based Immunosensor for Sensitive Label-Free Impedimetric Detection of SARS-CoV-2 Spike Protein. ACS applied materials & interfaces, PMID: 38814245
8, Brustoloni, CJ; et al. Systematic Study of Various Functionalization Steps for Ultrasensitive Detection of SARS-CoV-2 with Direct Laser-Functionalized Au-LIG Electrochemical Sensors. ACS applied materials & interfaces, PMID: 39231012
9, Asgharian, H; et al. Multi-Electrode Extended Gate Field Effect Transistors Based on Laser-Induced Graphene for the Detection of Vitamin C and SARS-CoV-2. ACS applied materials & interfaces, PMID: 39470169
HIS 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的 HIS 标签蛋白。该问题主要可以分为以下两个方面： 1、HIS 标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螫合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲
Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay
229E and OC43 of human coronavirus (HCoV). An alternative indirect immunoperoxidase assay (IPA) is herein described for the detection and titration of these viruses. Susceptible cells are inoculated with serial logarithmic dilutions of samples in a 96
蛋白标签（protein tag）是指利用DNA体外重组技术，与目的蛋白一起融合表达的一种多肽或者蛋白，以便于目的蛋白的表达、检测、示踪和纯化等。随着技术的不断发展，研究人员相继开发出了具有各种不同功能的蛋白标签。 一、 新型蛋白标签 蛋白标签应用极为广泛，但仍有两个问题不容忽视：（1）蛋白标签以DNA形式编码，它需要转录并翻译成蛋白后才能发挥作用，而这种作用方式本身就是一种缺陷。例如，常用的荧光蛋白标签，在显微镜下观察时，其显像不如人工合成的荧光基团好，但是这种人工合成的荧
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