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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
请询
- 英文名:
Candor Bioscience:LowCross® HRP
- 库存:
部分现货库存
- 供应商:
青木生物技术(武汉)有限公司
- 规格:
50 ml/125 ml/500 ml
| 规格: | 50 ml | 产品价格: | ¥1426.0 |
|---|---|---|---|
| 规格: | 125 ml | 产品价格: | ¥3174.0 |
| 规格: | 500 ml | 产品价格: | ¥11914.0 |
LowCross-Buffer® stands for superior reliability by antagonising assay interferences including HAMA-derived ones.
The efficient HAMA blocking activity of LowCross-Buffer® now also includes superior HRP-stabilization components. The result is an assay diluent for peroxidase conjugates with high reliability and convenience due to ready-to-use dilution.
文献引用:
- C. Mazziotta et al. (2021). Decreased IgG Antibody Response to Viral Protein Mimotopes of Oncogenic in Cell Polyomavirus in Sera From Healthy Elderly Subjects." Frontiers in Immunology (2021): 4206. M. Park et al. (2021).
- Valproic acid protects intestinal organoids against radiation via NOTCH signaling. Cell Biology International. doi.org/10.1002/cbin.11591 D. Johnson et al. (2021).
- Sensitive assay design for detection of anti-drug antibodies to biotherapeutics that lack an immunoglobulin Fc domain. Scientific Reports, 11(1), 1-12. V. D. Gonzalez et al. (2021).
- High-grade serous ovarian tumor cells modulate NK cell function to create an immune-tolerant microenvironment. Cell Reports, 36(9), 109632. D. Junker et al. (2021).
- Reduced serum neutralization capacity against SARS-CoV-2 variants in a multiplex ACE2 RBD competition assay. medRxiv. doi.org/10.1101/2021.08.20.21262328 M. Lehmann et al. (2021).
- Human small intestinal infection by SARS-CoV-2 is characterized by a mucosal infiltration with activated CD8+ T cells. Mucosal Immunology, 1-12. R. Kant et al. (2021).
- Serendipitous COVID-19 Vaccine-Mix in Uttar Pradesh, India: Safety and Immunogenicity Assessment of a Heterologous Regime. medRxiv. doi.org/10.1101/2021.08.06.21261716 C. F. Fotis et al. (2021).
- Robust SARS-COV-2 serological population screens via multi-antigen rules-based approach. Scientific reports, 11(1), 1-11. S. Mohandas et al. (2021). Comparison of the pathogenicity and virus shedding of SARS CoV-2 VOC 202012/01 and D614G variant in hamster model. BioRxiv. doi.org/10.1101/2021.02.25.432136
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文献和实验- C. Mazziotta et al. (2021). Decreased IgG Antibody Response to Viral Protein Mimotopes of Oncogenic in Cell Polyomavirus in Sera From Healthy Elderly Subjects." Frontiers in Immunology (2021): 4206. M. Park et al. (2021).
- Valproic acid protects intestinal organoids against radiation via NOTCH signaling. Cell Biology International. doi.org/10.1002/cbin.11591 D. Johnson et al. (2021).
- Sensitive assay design for detection of anti-drug antibodies to biotherapeutics that lack an immunoglobulin Fc domain. Scientific Reports, 11(1), 1-12. V. D. Gonzalez et al. (2021).
- High-grade serous ovarian tumor cells modulate NK cell function to create an immune-tolerant microenvironment. Cell Reports, 36(9), 109632. D. Junker et al. (2021).
- Reduced serum neutralization capacity against SARS-CoV-2 variants in a multiplex ACE2 RBD competition assay. medRxiv. doi.org/10.1101/2021.08.20.21262328 M. Lehmann et al. (2021).
- Human small intestinal infection by SARS-CoV-2 is characterized by a mucosal infiltration with activated CD8+ T cells. Mucosal Immunology, 1-12. R. Kant et al. (2021).
- Serendipitous COVID-19 Vaccine-Mix in Uttar Pradesh, India: Safety and Immunogenicity Assessment of a Heterologous Regime. medRxiv. doi.org/10.1101/2021.08.06.21261716 C. F. Fotis et al. (2021).
- Robust SARS-COV-2 serological population screens via multi-antigen rules-based approach. Scientific reports, 11(1), 1-11. S. Mohandas et al. (2021). Comparison of the pathogenicity and virus shedding of SARS CoV-2 VOC 202012/01 and D614G variant in hamster model. BioRxiv. doi.org/10.1101/2021.02.25.432136
CANDOR BIOSCIENCE: 关于ELISA板稳定性的技术探讨与比较
稳定剂,例如CANDOR的Antibody Stabilizer、HRP-Protector™和AP-Protector®,可以大大提高对照品和检测抗体-结合物的稳定性。本文以ELISAs为例,重点讨论固相稳定、表面包被捕获分子的活性维持的机理和解决方案。此外,本文概述的原理和解决方案也可以应用于其他包被表面,如聚苯乙烯或玻璃表面的实验应用,如侧向层析、亲和柱、基于微球的分析、免疫PCR或蛋白质分析等。 包被的捕获分子如抗体,或在抗原下降分析中分离的病毒蛋白,是可靠免疫分析的核心组成
数量的低亲和力结合也会导致高分析读数,产生特定的假阳性结果,或者高背景和坏的信噪比。一种潜在的方法是使用高浓度的分析稀释剂来减少这些有问题的中低亲和力相互作用的影响。然而,这些高浓度的条件也会对高亲和力结合产生负面影响,从而影响分析的正确信号。因此,高浓度缓冲液不是一个有用的选择。 减少低亲和力和中亲和力结合作用而不影响高亲和力结合的唯一方法是亲和力鉴别。15年前,CANDOR BIOSCIENCE的LowCross Buffer®产品成功引入了这种作用模式,此后消除了数百万样本中的干扰。如果用于
7.6,高背景或 AP 存在时推荐使用 C0905 荧光抗体专用稀释液 10ml 50 pH 7.2,不能加入血清 C0906 HRP稳定剂 10ml/100ml 50/350 pH7.2,无蛋白成分 *-所有稀释液在4℃保存抗体时,能稳定










