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Goat anti-Human IgA, IgG, and

IgM, HRP conjugate; 2 mL
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  • 询价
  • AP120P
  • 2026年01月08日
  • ELISA;Western Blotting
  • Goat
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      ELISA;Western Blotting

    • 宿主：

      Goat

    • 抗原来源：

      0

    • 适应物种：

      Human

    • 库存：

      大量

    • 是否单克隆：

      2

    • 规格：

      2 mL

    Species Reactivity Key Applications Host Format Antibody Type
    H ELISA, WB, IH Goat HRP Polyclonal Antibody
    Description:
    Goat anti-Human IgA, IgG, and IgM, HRP conjugate
    Background Information:
    IgG is the most abundant immunoglobulin comprising two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15% of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
    Specificity:
    The antibody reacts with human IgG, IgM and IgA. May also react with the light chains of other human immunogloibulins.
    Key Applications:
    • ELISA
    • Western Blotting
    • Immunohistochemistry
    Application Notes:
    EIA: 1:5,000-1:100,000.
    Western blots: 1:5,000-1:200,000.
    Immunohistochemistry: 1:500-1:5,000.

    Optimal working dilutions must be determined by the end user.
    Species Reactivity:
    Human
    Immunogen:
    Prepared from purified human IgG, IgM and IgA.
    Presentation:
    Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.
    Storage Conditions:
    Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
    Antibody Category:
    Secondary & Control Antibodies
    Antibody Sub-Category:
    Whole Immunoglobulin Secondary Antibodies
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    HRP
    Host:
    Goat
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    Prepared from purified human IgG, IgM and IgA. 详细描述见链接：http://www.millipore.com/catalogue/item/AP120P

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    图标文献和实验
    相关实验

    • 【交流】谁用过RD公司的HRP酶标羊抗鼠IgG试剂盒.

      我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06

    • Anti-P-CREB

      which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates

    • Western Blot/Anti-P-CREB

      % nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope).   Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000

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